| Author: | Jesper Pedersen (jsp at phage.dk) |
| History: |
2008-12-30 - Version 1: Based on the Stack_Normalizer plugin by Joachim Walter 2010-09-09 - Version 2: Uses ImageJ functions to measure mean and multiply frames in stead of getPixelValue and putPixel - These changes make the plugin smaller and about 4 times faster than version 1 - YAY!. |
| Requires: | ImageJ 1.32c or later |
| Source: | Stack_Deflicker.java |
| Installation: | Download Stack_Deflicker.class to the plugins/Stacks folder and restart ImageJ. |
| Description: |
The Stack_Deflicker calculates the average grey value for each frame and normalizes all frames so that they have same average grey level as a specified frame of the stack.
This plugin is very useful to remove flickering in movies caused by frame rates different from the frequency of AC used for the light-source that illuminate the scene.
An input value of -1 corresponds to the brightest frame while an input value of zero corresponds to the faintest frame.
If a region of the stack is selected the average frame intensity will be calculated from this region. See example of movies before and after deflickering below:
C. elegans swimming in M9 buffer on an agar plate. |
| See Also: | Stack Normalizer plugin and Process/Enhance Contrast command |
